EB 3530 - Cell Culture Lab for BioMolecular Engineering

• Get BSL2+ trained
• Perform cell culture using mammalian cell lines
• Prepare media and recognize the vital components of media and perform aseptic techniques imperative for primary and immortalized mammalian cells
• Conduct cell count, design growth curve experiments, and formulate conditions for optimal transient transfection of cells
• Identify machines used in cell culture
• Recognize critical components of the mechanism of how cell culture reagents work and impact of cell culture on cell engineering
• Handle calculations associated with cell culture and be able to use those in new situations
• Recognize that the field of cell culture needs constant practice and discipline
• Recognize the engineering application of cell culture to the design, analysis and control of chemical, physical, and/or biological processes
• Practice safety and ethics involved with the field of cell culture
• Recognize the hazards associated with these processes

• Basic knowledge on cell, biomolecules and metabolism from BI 102 , CH 223 , and EB 2240  

• Syllabus, introduction to cell culture
• Aseptic techniques/passaging counting and housekeeping cells
• SAFETY AND HAZARDS guest speaker from EHS department
• Industrial use of plant and animal cell culture
• Transfer of foreign DNA into animal and plant cells
• Applications and scale-up
• Design of the projects discussion
• Immunostaining

• Aseptic techniques/counting cells/scopes and passaging
• Senior design mini project: (select and perform 1)
• Design and establish a process of cell healing (scratch assays). Can be done on all available cell lines, including stem cells
• Design transformation of embryonic stem cells into a different kind. Can be done on available embryonic rabbit stem cells
• Design cellular differentiation of cells, changing them into a tissue and establishing the mechanical properties using a palpator. Can be done on all cell line