Dec 21, 2024  
2019-2020 Undergraduate Academic Catalog 
    
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2019-2020 Undergraduate Academic Catalog [ARCHIVED CATALOG]

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EB 2240 - Engineering Applications in Biochemistry

2 lecture hours 2 lab hours 3 credits
Course Description
Extensions of the principles of biochemistry are applied to biomolecular engineering. The course provides exposure to enzyme catalysis and kinetics, metabolic pathways, their regulation and associated bioenergetics, cell potential and microbial and H-fuel cell. The interplay of biochemistry, molecular biology, biomolecular and biochemical engineering problems is examined. Laboratory experiments reinforce the concepts from lectures, with an emphasis on applied methods. (prereq: EB 2001 , CH 201 , and either BI 102  or CH 223 )
Course Learning Outcomes
Upon successful completion of this course, the student will be able to:
  • Gain knowledge of engineering applications of biochemistry concepts
  • Perform experimentation related to biomolecular engineering, including hypothesis formulation, model development, measurements with positive and negative controls, and data analysis
  • Gain knowledge to design, analyze and control physical, chemical, and biological processes
  • Learn to apply basic science engineering concepts knowledge to new situations
Prerequisites by Topic
  • Material taught in CH 200 , CH 222  and CH 223  
  • Material taught in MA 136 , MA 137 , PH 2011 , BI 102 , and EB 2001  
  • Definitions and nomenclature of basic organic and biomolecules
  • Organic functional groups
  • Monomers of basic biomolecules, proteins, enzymes, nucleic acids, lipids, carbohydrates
  • Directionality of biomolecules, properties of biomolecules
  • Key points of metabolism
  • Nucleophiles and electrophiles, hydrophobicity and hydrophilicity
Course Topics
  • Introduction to the syllabus
  • Enzyme catalysis
  • Enzyme kinetics
  • Applications commercial/industrial use of proteins and enzymes
  • Regulations (enzymes) and applications
  • A great engineering example-the cell and applications
  • H-fuel cell and microbial fuel cell
  • Bioenergetics
  • Design of metabolism and aerobic metabolism
  • Photosynthesis and engineering aspects of metabolism
  • Designing of proteins and enzymes and applications
Laboratory Topics
  • Lab 1: Log notebook, good lab practices, dos and don’ts of LMPS
  • Lab 2: Fuel cells: H and microbial fuel cell
  • Lab 3: Diffusion measurement in a two-compartment model
  • Lab 4, 5, 6: Model trays for the electron pathway and energy transfer during respiration and photosynthesis
  • Lab 7: Measurement of enzymatic reaction rate
  • Lab 8: Measurement of enzymatic reaction rate with inhibitor
  • Lab 9: Fuel cell challenge design
Coordinator
Dr. Gul Afshan


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