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Mar 13, 2025
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BIE 3100 - Cell Culture and Tissue Engineering2 lecture hours 3 lab hours 3 credits
Course Description
Cell culture and tissue engineering are dynamic and rapidly growing fields. The course examines fundamental cell culture and cryopreservation techniques and cell/tissue engineering approaches. Course topics range from biosafety, mammalian cell culture, and bioreactors for cell and tissue culture to decellularization, bioprinting, and related bioethics. This course also provides extensive hands-on experience in cell culture and tissue engineering within a BLS-II setting. (prereq: BIE 2100 ) (quarter system prereq: EB 3410)
Course Learning Outcomes
Upon successful completion of this course, the student will be able to:
- Perform basic mammalian cell culture using animal cell lines
- Formulate and prepare cell culture media
- Identify materials and equipment used in cell culture
- Function in a BSL-II facility safely and efficiently
- Demonstrate laboratory skills related to basic cell and tissue engineering
- Design cell culture and engineering processes for various applications, including tissue engineering
Prerequisites by Topic
- Prokaryotes and eukaryotes cells
- Cell and tissue differences
Course Topics
- Aseptic techniques
- Types of cell culture, cell counting and passaging
- Mammalian cell cryopreservation methods and process
- Biosafety cabinets basics
- Biosafety and biosecturity
- Stem cell and cell differentiation
- Cell and engineering basics
- Bioreactors for cell and tissue culture
- Vascularization and decellularization
- Bioprinting
- Ethics for cell and tissue engineering
Laboratory Topics
- BSL-II lab training
- Microscopes and hemocytometers
- Mammalian cell culture and passaging
- Mammalian cell cryopreservation
- Cell engineering
- ECM and scaffolds
- Decellularization
- Engineer tissue constructs
Coordinator
Dr. Wujie Zhang
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