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Apr 17, 2024
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EB 3820 - Molecular Biology for Engineering3 lecture hours 0 lab hours 3 credits Course Description This course builds upon the current molecular biology foundation and adds both breadth and depth on this topic. New modern molecular biology techniques and contemporary issues are discussed along with problem-based applications. (prereq: BI 2020 and junior standing) Course Learning Outcomes Upon successful completion of this course, the student will be able to:
- Apply the biotechnology tools previously learned toward applications in biomolecular engineering
- Discuss new developments in a variety of biotechnology fields in regard to the biomolecular engineering field
- Discuss the components necessary for founding a biotechnology company
- Apply their knowledge of biology, chemistry, and biotechnology to solve basic problems in the biotechnology and biomolecular engineering field
Prerequisites by Topic
- Microbial cell structure
- Microbial growth
- Microbial genetics
- Viruses, viroids and prions
- Microbial mathematics/lab tools
- Food microbiology
- Water and sewage treatment
- Environmental microbiology
- Bioremediation
- Future microbial challenges
Course Topics
- Review basic information on DNA, RNA, and proteins
- DNA techniques: cloning and vectors
- DNA techniques: PCR
- DNA techniques: Nucleic acid hybridization - southern blots and in-situ hybridization
- RNA techniques: RNA isolation and purification
- RNA techniques: nucleic acid hybridization - northern blots
- Molecular tools: labeled tracers
- Protein techniques: genome editing
- Protein techniques: DNA-protein and protein-protein interactions
- Whole organism techniques: knock-in, knock-out, and transgenic mice
Coordinator Dr. Eryn Hassemer
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