Aug 18, 2022  
2020-2021 Undergraduate Academic Catalog 
2020-2021 Undergraduate Academic Catalog [ARCHIVED CATALOG]

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EB 2410 - Principles of Biotechnology

2 lecture hours 2 lab hours 3 credits
Course Description
Principles of cell biology, biochemistry, and molecular biology are summarized in the context of biomolecular engineering. Examples of molecular, biochemical and industrial based processes are presented. Lectures focus on the theory of critical techniques that are the backbone of the biotechnological molecular industry. Students have opportunities for hands-on application of techniques during lab sessions. History, ethics and societal impact of biotechnology are discussed. (prereq: BI 2020 )
Course Learning Outcomes
Upon successful completion of this course, the student will be able to:
  • Discuss the history, impacts, and implications of biotechnology
  • Discussion pros and cons of the different aspects of the field
  • Discuss the underlying principles of several bio-techniques
  • Perform several molecular level biotechnology experimental techniques including hypothesis formulation and measurements with positive and negative controls
  • Perform data analysis using knowledge of basic sciences and engineering
  • Practice at least three different techniques of biotechnology independently
  • Identify several biomolecular engineering applications of biotechnology
  • Integrate molecular knowledge into analysis and design experiments

Prerequisites by Topic
  • Basic terminology of nucleic acids, proteins, and other biomolecules

Course Topics
  • Syllabus, history, safety and ethics in biotechnology (1 class)
  • Lifelong learning in biotechnology (1 class)
  • Different types of biotechnology (1 class)
  • Impact of biotechnology on engineering (1 class)
  • Basic skills - doing, speaking, thinking biotech (2 classes)
  • Isolating and manipulating biomolecules, DNA, proteins (2 classes)
  • Genetic engineering/cloning (3 classes)
  • Transformations/fermentations (2 class)
  • Operons and transformations (1 class)
  • Infections/transfections (1 class)
  • Polymerase chain reaction (2 classes)
  • Forensics (2 classes)
  • Design project presentation and reevaluation (outside class time half a day Saturday)
  • Advanced topics (if time allows)
  • Review

Laboratory Topics
  • Intro to biotechnology methods
    • Setting up a legal scientific notebook
    • Laboratory safety and ethics
  • Chemistry needed for biotechnology methods
    • Measuring very small volumes
    • Measuring mass
    • Making solutions
    • Making dilutions
    • Basic biotechnology calculations
  • Role of biomolecules in biomolecular engineering
    • Understanding design of two strands of DNA
    • DNA resolving gels
    • DNA isolation
    • Quantitation of DNA via gel electrophoresis
    • Quantitation of DNA via spectrophotometer
  • Manipulations of biomolecules models for molecular engineering
    • Transformation of bacteria with plasmid (model of switches at work)
    • Restriction analysis of pre cut DNA sequence (phage)
    • Crime scene MSOE - DNA fingerprinting
    • Visiting a crime lab
    • Data analysis
  • Amplification models for molecular engineering
    • Polymerase chain reaction
    • Extended activities

Dr. Eryn Hassemer

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