Dec 05, 2019  
2017-2018 Undergraduate Academic Catalog 
2017-2018 Undergraduate Academic Catalog [ARCHIVED CATALOG]

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EB 3530 - Cell Culture Lab for BioMolecular Engineering

1 lecture hours 4 lab hours 3 credits
Course Description
The course presents valuable hands-on experience in cell culturing aseptic techniques and their applications in industrial manufacturing and bio-manufacturing. Basics of cell culture techniques, controls and conditions, safety and hazards, types of cell culture, cell environment, cryopreservation and storage of cell lines, good cell banking procedures, alternative cell culture systems, process protocols, bioreactor design and operation, cell growth models and emerging technologies will be discussed and practiced. (prereq: EB 3410 )
Course Learning Outcomes
Upon successful completion of this course, the student will be able to:
  • Perform cell culture using mammalian cell lines
  • Prepare media and recognize the vital components of media and perform aseptic techniques imperative for primary and immortalized mammalian cells
  • Conduct cell count, design growth curve experiments and formulate conditions for optimal transient transfection of cells
  • Identify machines used in cell culture
  • Recognize critical components of the mechanism of how cell culture reagents work and impact of cell culture on cell engineering
  • Handle calculations associated with cell culture and be able to use those in new situations
  • Recognize that the field of cell culture needs constant practice and discipline
  • Recognize the engineering application of cell culture to the design, analysis and control of chemical, physical, and/or biological processes
  • Practice safety and ethics involved with the field of cell culture
  • Recognize the hazards associated with these processes

Prerequisites by Topic
  • Basic knowledge on cell, biomolecules and metabolism from BI 102 , CH 223  and EB 2240  

Course Topics
  • Syllabus, Introduction to Cell Culture
  • Aseptic Techniques/Passaging Counting and Housekeeping Cells
  • SAFETY AND HAZARDS Guest Speaker from EHS department
  • Industrial use of plant and animal cell culture
  • Transfer of foreign DNA into animal and plant cells
  • Applications and Scale-UP
  • Design of the projects discussion
  • Immunostaining

Laboratory Topics
  • Aseptic Techniques/Counting Cells/scopes and Passaging
  • Senior Design Mini Project: (Select and Perform 1)
  • Design and establish a process of cell healing (Scratch Assays). Can be done on all available cell lines, including stem cells
  • Design transformation of Embryonic Stem cells into a different kind. Can be done on available embryonic rabbit stem cells
  • Design cellular differentiation of cells, changing them into a tissue and establishing the mechanical properties using a palpator. Can be done on all cell line

Gul Afshan

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