Dec 05, 2019  
2017-2018 Undergraduate Academic Catalog 
2017-2018 Undergraduate Academic Catalog [ARCHIVED CATALOG]

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EB 2240 - Engineering Applications in Biochemistry

2 lecture hours 2 lab hours 3 credits
Course Description
Extensions of the principles of biochemistry are applied to biomolecular engineering. The course provides exposure to enzyme catalysis and kinetics, metabolic pathways, their regulation and associated bioenergetics, cell potential and microbial and H-fuel cell. The interplay of biochemistry, molecular biology, biomolecular and biochemical engineering problems is examined. Laboratory experiments reinforce the concepts from lectures, with an emphasis on applied methods. (prereq: BI 102 , CH 223 )
Course Learning Outcomes
Upon successful completion of this course, the student will be able to:
  • Gain knowledge of engineering applications of biochemistry concepts
  • Perform experimentation related to biomolecular engineering, including hypothesis formulation, model development, measurements with positive and negative controls, data analysis
  • Gain knowledge to design, analyze and control physical, chemical and biological processes
  • Learn to apply basic science engineering concepts knowledge onto new situations

Prerequisites by Topic
  • Material taught in CH 200  , CH 222   and CH 223  
  • Material taught in MA 136 ,MA 137 , MA 231  ,PH 2010  , BI 102   and EB 2000  
  • Definitions and nomenclature of basic organic and biomolecules
  • Organic Functional groups
  • Monomers of basic biomolecules, proteins, enzymes, nucleic acids, lipids, carbohydrates
  • Directionality of biomolecules, properties of biomolecules
  • Key points of metabolism
  • Nucleophiles and Electrophiles, Hydrophobicity and hydrophilicity

Course Topics
  • Introduction to the Syllabus, Pre Exam
  • Enzyme Catalysis
  • Enzyme Kinetics
  • Applications Commercial/Industrial use of Proteins and Enzymes
  • Regulations (Enzymes) and Applications
  • A Great Engineering Example-the Cell and Applications
  • H-Fuel Cell and Microbial Fuel Cell
  • Bioenergetics
  • Design of Metabolism and Aerobic Metabolism
  • Photosynthesis and Engineering Aspects of Metabolism
  • Designing of Proteins and Enzymes and Applications

Laboratory Topics
  • Lab 0: Log notebook, good Lab practices, dos and donts of LMPS
  • Lab 1: Diffusion Measurement in a Two-Compartment Model
  • Lab 2: Measurement of Enzymatic Reaction Rate
  • Lab 3: Dissociation of Double Stranded Polynucleotides
  • Lab 4: Fuel Cells: H and Microbial Fuel Cell
  • Lab 5: Model trays for the Electron Pathway and Energy Transfer during Respiration and Photosynthesis
  • Lab 6: Fuel Cell Challenge Design

Gul Afshan

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