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Dec 21, 2024
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CH 373 - Advanced Biotechnology2 lecture hours 2 lab hours 3 credits
Course Description
Advance techniques of biotechnology are introduced. The core of this elective course covers concepts, procedures and techniques used in the areas of advanced biotechnology. Lab activities provide hands-on practice on concepts taught in lecture. (prereq: CH 200 , CH 200A or CH 200B )
Course Learning Outcomes
Upon successful completion of this course, the student will be able to:
- Understand and perform several advance level biotechnology techniques
- Understand the intellectual manipulations to work through the techniques
- Understand theoretical concept of the cell culture
- Use the proper terminology for several cell culture techniques
- Independently perform few basic sterile techniques of the bacterial culture
- Present a talk and lead a discussion session on the new ideas in the field
Prerequisites by Topic
- Eukaryotic and Prokaryotic cells
Course Topics
- Cell growth and sterile techniques
- Bacterial culture
- Technology: Advantages and Applications
- Propagation and maintenance of a mammalian (eukaryotic) cell line
- Extraction and purification of mRNA from eukaryotic cells
- Transfection of mammalian cell lines
- Detection of mycoplasma in the mammalian cells
- Expression of a commercially available green fluorescent protein
Laboratory Topics
- Sterile Techniques
- Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (PAGE)
- Blue Staining of Proteins
- Western Blotting and Immuno-detection
- Oligonucleotide Design by Computer
- Polymerase chain reaction (PCR)
- Quantitation of Proteins
- Internet Gene Bank Search
Coordinator
Gul Afshan
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