Dec 05, 2019  
2017-2018 Undergraduate Academic Catalog 
2017-2018 Undergraduate Academic Catalog [ARCHIVED CATALOG]

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EB 3200 - Bioanalytical Instrumentation

2 lecture hours 2 lab hours 3 credits
Course Description
This course introduces bioprobing, bioanalyzing and high throughput data technology as applied to the field of biomolecular engineering. Mass spectroscopy, Fourier-transform infrared spectroscopy (FTIR), electron microscopy (EM) and atomic force microscopy (AFM) are introduced. High-throuput analytical techniques are studied through the use of plate reader and PalpatorTM. Laboratory experiments provide hands on experience and reinforce material taught in lecture. (prereq: PH 2031 , EB 2410 )
Course Learning Outcomes
Upon successful completion of this course, the student will be able to:
  • Identify key components of several machines used for probing and analysis of biomolecules
  • Demonstrate understanding of concepts, principles of operations and applications of the field
  • Recognize the vital components of sample preparation for the probing and analysis
  • Distinguish between the principles and constraints of high and low throughput
  • Recognize that bioprobing and bioanalysis need constant practice and discipline
  • Practice safety and ethics involved with the field of bioprobing and bioanalysis
  • Recognize and list the hazards associated with the field of bioprobing and bioanalysis

Prerequisites by Topic
  • Biomolecules structure and function. Physics of mechanics, Physics of electricity and magnetism and modern physics

Course Topics
  • Atomic Force Microscopy (AFM)-the basics
  • AFM-beyond the basics - using AFM to characterize individual biomolecules, such as DNA
  • Fourier-Transform Infrared Spectroscopy (FT-IR). Theoretical foundations of vibrational spectroscopy. Principles of operation of IR and FT-IR
  • FT-IR in biomolecular engineering: spectra of functional groups, application of FT-IR to secondary structure of proteins
  • Safety, hazards, discipline, sample prep
  • Principles of plate reading/immunolabeling
  • Applications/constrains of plate reading/immunolabeling
  • Electron microscopy-principles and applications
  • Mass spectroscopy-principles and applications
  • Basic principles of operation of PalpatorTM and the range of its possible applications in the biomolecular engineering field
  • Using the PalpatorTM for high-throughput cellular characterization and cellular treatment efficiency, i.e. pharmaceutical efficacy or toxicity testing

Laboratory Topics
  • Laboratory safety, hazards, discipline
  • Hands on AFM-contact and tapping mode imaging of objects of known morphology. Basic use of nanoscope
  • Analysis software
  • Hands on AFM. Preparation of biological samples: DNA on mica. Contact and tapping mode AFM of DNA on mica
  • Protein sample preparation. Acquiring spectra of buffer and BSA in buffer
  • Analysis of FT-IR spectra of BSA in buffer and determination of changes in the secondary structure of proteins
  • Hands on sample prep and Plate reader principle and application
  • Tour to Mass Spec facility at MCW
  • Hands on sample prep and PalpatorTM application to cell characterization

Matey Kaltchev

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