CH 2260 - Biochemistry for Life Sciences

• Identify alpha amino acids, write reactions of peptide bond formation, describe proteins in terms of size, function, fibrous, globular, structural hierarchy, explain hydrolysis and denaturation
• Sescribe functions of carbohydrates, classify carbohydrates, describe stereoisomerism, write reactions of oxidation and glycoside formation, describe structure and use of polysaccharides
• Classify lipids, draw structures of fats and oils, describe their chemical properties, describe phosphoglycerides, sphingolipids, describe major features of sphingolipids, identify steroids
• Describe enzymes and how they work, factors affecting enzyme activity, explain enzyme inhibition, describe regulation of enzymes, recognize importance of enzymes in diseases
• Identify nucleotides, describe structure of DNA, outline replication process, describe transcription and translation, explain how genetic code functions, describe mutations
• Describe nutritional requirements, vitamins and minerals, outline stages of metabolism, role of ATP, coenzymes, describe metabolism of glucose in details, hormonal control of metabolism
• Outline fat metabolism, describe medical conditions of ketonemia, ketonuria, ketosis, ketoacidosis

• None

• Amino acids, peptides, protein function, primary, secondary, tertiary, quaternary structures of proteins, protein hydrolysis and denaturation
• Classes of carbohydrates, properties of mono, di and polysaccharides, stereochemistry of carbohydrates
• Classification of lipids, structure chemical properties of fats and oils, phosphoglycerides, sphingolipids, trans fatty acids, LDL, HDL, cholesterol, biological membranes, steroids, bile salts, steroid hormones, prostaglandins
• Enzyme nomenclature, enzyme co-factors, mechanism of enzyme action, factors affecting enzyme action, enzyme inhibition, enzyme regulation, medical applications of enzymes
• Nucleic acids, structure of DNA and RNA, DNA replication, transcription, translation, genetic code, mutations
• Nutritional requirements, macro and micronutrients, catabolism of food, ATP, coenzymes, blood glucose, glycolysis, citric acid cycle, oxidative phosphorylation, glycogen metabolism, hormonal regulation
• Blood lipids, beta oxidation of fatty acids, ketone bodies and pathological conditions that cause their overproduction

• Experiment #1: Properties of vitamins (439)
• Experiment #2: Chemistry of amino acids (pg 397)
• Experiment #3: Peptides and Proteins (pg.409)
• Experiment #4: Tests for carbohydrates-A, B, C, D (pg. 275)
• Experiment #5: Tests for carbohydrates- C, E and F (pg. 275) and diastase activity
• Experiment #6: Lipids (pg. 319)
• Experiment #7: Enzymes (pg. 423)
• Experiment #8: DNA components and extraction (pg. 309)
• Experiment #9: Analysis of Urine (pg. 477)